Culture media and medium- microbiology learn it easy with stencildent

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 CULTURE MEDIUM AND CULTURE MEDIA 

CULTURE MEDIUM:

DEFINITION: A chemically prepared solid /liquid substance used to support the growth of microorganism

Uses : 

  1. To provide nutrition
  2. To provide space
  3. To provide growth
  4. To increase population
  5. To handle stress
  6. To differentiate species 


COMPONENTS:

1) NITROGEN SOURCE- Amino,protein extract,peptone

2) Growth factor- Blood serum,meat,yeast extract

3)Energy- Sugar,alcohol,carbohydrate

4)Buffer - Maintain ph ,phosphate,acetate 

5) Salt- Enhance growth,phosphate ,sulfate,magnesium



SELECTIVE AGENTS:

  • CHEMICAL
  • ANTIBIOTICS
  • INDICATOR DYE

GELLING AGENT : 

  • Act as solidifying agent 
  • agar ,gelatin- agents
CULTURE MEDIA 

  • Culture media is required to grow organism from infected material to identify the causative agent
  • It contains: water,vitamin,mineral,growth factor,blood,serum,agar,peptone  
TYPES :
  1. SIMPLE
  2. COMPLEX
  3. SPECIAL: ENRICHED,ENRICHMENT,SELECTIVE,DIFFERENTIAL,INDICATOR,TRANSPORT,SUGAR 
  4. SYNTHETIC
  5. ANAEROBIC : DEHYDRATE 


SIMPLE:
  • Nutrient broth - peptone water+meat extract
  • glucose broth- peptone water+glucose
  • nutrient agar- nutrient broth +agar ( 2-3%)
complex media : 
  • all media other than simple media
synthetic media: 
  • they are prepared from pure chemical
media                                          use 
  • nutrient broth                  routine culture
  • glucose broth                  blood culture
  • nutrient agar                routine culture 
SPECIAL MEDIA: 
BLOOD MEDIA - Nutrient agar +sheep blood
eg- streptococcus -require blood for growth
CHOCOLATE AGAR;
Used in haemophilus influenza
ENRICHMENT MEDIA 
1) SELECTIVE F BROTH
Culture of faeces for salmonella and shigella
2) cooked meat broth /robertson cooked meat broth
culture of anaerobic bacteria
SELECTIVE MEDIA : 
  1. Deoxycholate citrate - culture of salmonella ,shigella
  2. bile salt agar - culture of vibrio cholerae





INDICATOR MEDIA 
Macconkey's medium- culture of gram negative bacilli
TRANSPORT MEDIA:
  • They are used for the transport of the clinical sample suspected to contain delicate organism or when the delay is expected while transporting the sample
  • Simpler terms from site of collection to laboratory 
  • Condition in the media is in such a way that they don't multiply within the collected container 
  • This also prevents drying of sample and maintain viability of the organism in the sample
  • Examples: stuart medium for neisseria species,
  • venkatraman ramakrishnan medium -vibrio cholerae
AIM OF THIS POST:
Today we started from learning about culture medium ,the components present then we moved on into culture media and under which we saw about the different types of culture media .hope this post would have been informative if this post helped you do let me know in the comment section below .
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