STENCILDENT

                       PACKED CELL VOLUME   Its also called hematocrit Volume occupied by red cell when sample of anticoagulated blood is centrifuged It indicates relative proportion of red cell to plasma It is expressed as percentage of original volume of blood or as decimal fraction USES: Detection of presence /absence of anemia or polycythemia Estimation of red cell indices (mean cell volume +mean corpuscular Hb concentration) Checking accuracy of Hb value (Hb in g/dl *3=PCV) METHODS: Two methods for estimation Macro(WINTROBE),MICRO(MICROHEMATOCRIT) Micro method is preferred : Rapid Convenient Small amount of blood After centrifugation of anticoagulated three zones can be seen above downward: Plasma Buffy coat Packed cell Smear from puffy coat for demonstration of lupus erythematosus, malaria parasite and immature cells plasma IS usually Straw coloured  If colorless-IRON DEFICIENCY PINK-HEMOGLOBINEMIA YELLOW-JAUNDICE MILKY-HYPERTRIGLYCERIDEMIA SIGNIFICANCE: In anemia :Packed cell vo

                   AGRANULOCYTOSIS Lack of granulocyte  Absolute neutrophil count (less than 100 per microliter) Rare condition Suppressed immune system CAUSES: May be inherited or genetic Drugs: Anti-thyroid ,Anti-psychotic ,antibiotic Exposure to toxic substance such as ARSENIC ,MERCURY Aplastic anemia SYMPTOM : Sore throat and mouth Fever ,chill Fatigue  Headache Sweating  Swollen gland RISK: If left untreated result in sepsis Male and female both at equal risk Certain drug  DIAGNOSIS: Blood test Neutrophil count less than 100 per microliter of blood Bone marrow biopsy TREATMENT: Change of drug Transfusion of granulocyte